Improvement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screening

The Journal of General and Applied Microbiology

Zhi Nan Xu¹  Zhi Hua Jin¹  Jiang Ping Lin¹  Pei Lin Cen¹ 

[1] Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University

关键词: Amycolatopsis mediterranei;    p-hydroxybenzoic acid;    propionate;    rational screening;    rifamycin B;    tryptophan;   

DOI  :  10.2323/jgam.48.329

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

An industrially applied rifamycin B-producing strain, Amycolatopsis mediterranei XC 1-02, was used for further screening. A special mutation and screening procedure was adopted to select a strain, which can alleviate the inhibition caused by both aromatic amino acid and p-hydroxybenzoic acid in the pathway of rifamycin B biosynthesis as well as enhance the production of propionate, one of the precursors of rifamycin B biosynthesis. By the above methods, a strain A. mediterranei XC 9-25 was obtained, and its rifamycin B productivity in shaking flask reaches 10 g/L, which is 2.38 times higher than that of the ancestral strain XC 1-02. The productivity of rifamycin B fed-batch fermentation in 60,000 L fermentor with A. mediterranei XC 9-25 reached 19.11 g/L.

【 授权许可】

Unknown