期刊论文详细信息
The Journal of General and Applied Microbiology
The effect of sodium acetate on the growth yield, the production of L- and D-lactic acid, and the activity of some enzymes of the glycolytic pathway of Lactobacillus sakei NRIC 1071T and Lactobacillus plantarum NRIC 1067T
Takao Iino1  Tai Uchimura1  Kazuo Komagata1 
[1] Laboratory of General and Applied Microbiology, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture
关键词: effect of sodium acetate;    D-lactate dehydrogenase;    L-lactate dehydrogenase;    lactate racemase;    Lactobacillus plantarum;    Lactobacillus sakei;    type of stereoisomers of lactic acid;   
DOI  :  10.2323/jgam.48.91
学科分类:微生物学和免疫学
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation
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【 摘 要 】

The effect of sodium acetate was studied on the change of the growth yield, the production of L- and D-lactic acid, and the activity of lactate dehydrogenases (LDHs; L-lactate dehydrogenase [EC 1.1.1.27, L-LDH] plus D-lactate dehydrogenase [EC 1.1.1.28, D-LDH]), fructose-1, 6-bisphosphate aldolase [EC 4.1.2.13, FBP-aldolase], and phosphofructokinase [EC 2.7.1.11, PFK] of Lactobacillus sakei NRIC 1071T and Lactobacillus plantarum NRIC 1067T. The growth yield of L. sakei NRIC 1071T was increased 1.6 times in the presence of sodium acetate compared with its absence. The activity of LDHs in L. sakei NRIC 1071T and L. plantarum NRIC 1067T was retained longer under the addition of sodium acetate in the reaction mixture. As a result, these strains produced much more lactic acid in the presence of sodium acetate compared with its absence. Furthermore, the activity of L-LDH in L. sakei NRIC 1071T cultivated in the presence of sodium acetate increased three times or more compared with the activity of the cells cultivated in its absence. Consequently, the type of stereoisomers of lactic acid produced by L. sakei shifted from the DL-type to the L-type because the ratio of L-lactic acid to D-lactic acid produced became larger with the addition of sodium acetate to culture media. This phenomenon was not observed in L. plantarum NRIC 1067T. Further, the participation of lactate racemase is discussed from the viewpoint of the production of D-lactic acid by L. sakei.

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