Polyphasic approaches to the identification of predominant polyphosphate-accumulating organisms in a laboratory-scale anaerobic/aerobic activated sludge system

The Journal of General and Applied Microbiology

Akira Hiraishi²  Yoshitaka Matsuo³  Shin Onda¹  Susumu Takii¹ 

[1] Department of Biological Sciences, Tokyo Metropolitan University;Department of Ecological Engineering, Toyohashi University of Technology;Department of Civil Engineering, Chuo University

关键词: activated sludge;    Candidatus Accumulibacter phosphatis;    denaturing gradient gel electrophoresis;    fluorescence in situ hybridization (FISH);    phosphate removal;    polyphosphate;    quinone profile;   

DOI  :  10.2323/jgam.48.43

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

By combination of denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA (PCR-DGGE), quinone profiling, and 16S rRNA-targeted fluorescence in situ hybridization (FISH), a polyphosphate-accumulating organism (PAO) responsible for phosphate (P)-removal was identified in activated sludge with high P-removal ability from a laboratory-scale anaerobic/aerobic continuous flow reactor. The DNA fragment from the most dense band on the DGGE gel was closely related to that of ‘Candidatus Accumulibacter phosphatis’ (β-Proteobacteria). Quinone profiling also suggested the predominance of β-Proteobacteria. FISH with a specific oligonucleotide probe designed for the sequence showed that the targeted bacterium was dominant in the activated sludge, and the accumulation and consumption of polyphosphate were observed by dual staining with 4′,6-diamidino-2-phenylindole. The bacterium was concluded to be the responsible PAO in the reactor. However, when the P-removal ability per cell slightly decreased, the dominance of the PAO greatly diminished in the activated sludge. Such sludge might be dominated by other types of PAOs.

【 授权许可】

Unknown