Phylogenetic identification of n-alkane assimilating Candida yeasts based on nucleotide divergence in the 5′ end of LSU rDNA gene

The Journal of General and Applied Microbiology

Mami Arie¹  Keizo Furuhashi¹  Hitoshi Matsuda¹  Masamichi Takagi² 

[1] Pharmaceutical and Biotechnology Laboratory, Japan Energy Corporation;Tokyo University of Agriculture and Niigata College of Pharmacy

关键词: Ascomycetous yeasts;    Candida maltosa;    Candida tropicalis;    Candida viswanathii;    n-alkane;    phylogeny;    ribosomal DNA;   

DOI  :  10.2323/jgam.46.257

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Phylogenetic relationships of several species within the n-alkane assimilating Candida yeasts were investigated by using characters from the nucleotide sequence of the variable D1/D2 region at the 5′ end of a large-subunit (26S) ribosomal DNA (rDNA) gene. First the nucleotide sequences of D1/D2 domain of Candida sp. 1098 (formerly identified as C. tropicalis 1098) and its dicarboxylic acid-producing-mutant strain M1210 were investigated. These two nucleotide sequences were identical and lacked only one base pair compared with that of C. maltosa CBS 5611 (type strain), and they were identified as C. maltosa. We then showed that C. maltosa IFO 1978 (formerly identified as C. cloacae) and C. maltosa IFO 1975 (formerly identified as C. subtropicalis) had the same nucleotide sequence and had only one base pair substitution compared with C. maltosa CBS 5611 (type strain), which is consistent with conventional classification. We also found that another widely studied n-alkane assimilating Candida yeast, C. tropicalis pk233, to be C. viswanathii.

【 授权许可】

Unknown