Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus

The Journal of General and Applied Microbiology

Tomoko Omura²  Yoshito Sadaie¹  Keiichi Goto²  Yukihiko Hara² 

[1] Radioisotope Center, National Institute of Genetics;Laboratory of QC and Food Analysis, Food Research Institute, Mitsui Norin Co., Ltd.

关键词: Bacillaceae;    genus Bacillus;    grouping;    identification;    16S rRNA gene;   

DOI  :  10.2323/jgam.46.1

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5′ end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.

【 授权许可】

Unknown