Production of alkaline protease by a genetically engineered Aspergillus oryzae U1521

The Journal of General and Applied Microbiology

Warin Samarntarn¹  Supaporn Cheevadhanarak¹  Morakot Tanticharoen¹ 

[1] Biotechnology Division, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi

关键词: alkaline protease;    Aspergillus oryzae;   

DOI  :  10.2323/jgam.45.99

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The production of alkaline protease of Aspergillus oryzae U1521 was examined in liquid culture. In a culture of defatted soybean only, it gave satisfactory enzyme yields at 584,000 U/g defatted soybean. When various carbohydrates were supplemented, enzyme production was significantly increased. An increase in production by lactose was the most marked. Enrichment with casitone or casein increased productivity, but not cornsteep solid. Media formulation (g/L) of defatted soybean 10, lactose 5, casitone 1, and KH2PO45 enhanced alkaline protease production by A. oryzae U1521 to a maximum of 1,410,000 U/g defatted soybean. Scaling-up experiments indicated the flask-scale results could be reproduced at 40 g of substrate in 5-L fermenter. The enzyme activity was maximum between pH 8–9 and at a temperature of 45°C.

【 授权许可】

Unknown