Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan

The Journal of General and Applied Microbiology

Akira Hiraishi²  Mitsuru Iwasaki²  Kenji Inagaki³  Noriaki Kishimoto¹  Yasuhide Tanimoto³  Hidehiko Tanaka³ 

[1] Faculty of Food Culture, Kurashiki Sakuyo University;Department of Ecological Engineering, Toyohashi University of Technology;Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

关键词: Alicyclobacillus;    hot spring;    phylogeny;    16S rDNA;    thermoacidophiles;   

DOI  :  10.2323/jgam.43.295

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Three strains of thermophilic-acidophilic bacteria isolated previously from different hot springs in Japan were characterized by molecular genetic methods. The strategy taken involved PCR amplification, sequencing and restriction pattern analysis of 16S rDNA, 16S-23S rDNA spacer polymorphism analysis and genomic DNA-DNA hybridization. A phylogenetic analysis based on 16S rDNA sequences showed that the new thermoacidophilic isolates formed a genetically coherent group at the species level and fell into a major cluster together with members of the genera Alicyclobacillus and Sulfobacillus with A. acidocaldarius and A. acidoterrestris as their closest relatives. The levels of binary sequence similarity between the isolates and the two Alicyclobacillus species were 97.6 to 97.9%, values considered low enough to warrant placement of the isolates in a distinct species of the genus Alicyclobacillus. The 16S rDNA restriction pattern analysis, but not 16S-23S rDNA spacer polymorphism analysis, was useful for differentiating the isolates from the established Alicyclobacillus species. DNA-DNA hybridization assays demonstrated a distinct phylogenetic position of our isolates as a genospecies within the genus Alicyclobacillus. On the basis of these results, the thermoacidophilic isolates should be classified into a new species of Alicyclobacillus. The results of this study suggest that this new genospecies of Alicyclobacillus is widely distributed in hot springs in Japan.

【 授权许可】

Unknown