The Journal of General and Applied Microbiology | |
Growth phase-dependent transcription of emrKY, a homolog of multidrug efflux emrAB genes of Escherichia coil, is induced by tetracycline | |
Katsuhide Yamasaki2  Ryutaro Utsumi2  Akinori Katoh2  Mayu Yamamoto2  Sachiko Yoshioka2  Hideaki Tagami1  Motoki Furue2  Hiroji Aiba1  Kanehisa Yamamoto2  Hiroyuki Tanabe2  | |
[1] Department of Molecular Biology, Faculty of Science, Nagoya University;Department of Agricultural Chemistry, Faculty of Agriculture, Kinki University | |
关键词: efflux pump; Escherichia coli; multidrug resistance; tetracycline; | |
DOI : 10.2323/jgam.43.257 | |
学科分类:微生物学和免疫学 | |
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation | |
【 摘 要 】
The genes emrK and emrY were found between genes dsdA and evgA at 51min on the Escherichia coli chromosome and form an operon. EmrK and EmrY are 50.4 and 63.3% identical in amino acid sequences to EmrA and EmrB, respectively, which together make up a multidrug resistant pump. To show that the emrKY operon can be expressed, we cloned the promoter with pMC1403 and constructed an emrK-lacZ' protein fusion plasmid, pMKD1. In E. coli MC4100 containing pMKD1, its expression was increased in the presence of a subinhibitory concentration of tetracycline, chloramphenicol or salicylate, but not by carbonylcyanide m-chlorophenylhydrazone, nalidixic acid or kanamycin. Furthermore, we have shown that emrKY transcription dependent on the growth phase is actually induced by tetracycline using a S1 nuclease protection assay.
【 授权许可】
Unknown
【 预 览 】
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