Biodegradation of the mixture of 2, 4, 6-trichlorophenol, 4-chiorophenol, and phenol by a defined mixed culture

The Journal of General and Applied Microbiology

Sung-Talk Lee²  Hee Sung Bae²  James M. Lee¹ 

[1] Department of Chemical Engineering, Washington State University;Department of Biological Sciences, Korea Advanced Institute of Science and Technology

关键词: Arthrobacter sp. CPR706;    4-chlorophenol;    degradation;    immobilization;    phenol;    Pseudomonas sp. TCP114;    2;    4;    6-trichlorophenol;   

DOI  :  10.2323/jgam.43.97

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Two new strains, Pseudomonas sp. TCP114 degrading 2, 4, 6-trichlorophenol (TCP) and Arthrobacter sp. CPR706 degrading 4-chlorophenol (4-CP), were isolated through a selective enrichment procedure. Both strains could also degrade phenol. The degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. For example, when all three components (TCP, 4-CP, and phenol) were present in the medium, a pure culture of CPR706 could not degrade any of the components present. This restriction on degradability could be overcome by employing a defined mixed culture of the two strains. The mixed culture could degrade all three components in the mixture through cooperative activity. It was also demonstrated that the mixed culture could be immobilized by using calcium alginate for the semi-continuous degradation of the three-component mixture. Immobilization not only accelerates the degradation rate, but also enables reuse of the cell mass several times without losing the cells' degrading capabilities.

【 授权许可】

Unknown