The rpoD1 gene of Synechococcus sp. strain PCC 7942 encodes the principal sigma factor of RNA polymerase

The Journal of General and Applied Microbiology

Kan Tanaka¹  Susumu Masuda¹  Yoshinori Sugimoto¹  Hideo Takahashi¹ 

[1] Institute of Molecular and Cellular Biosciences, The University of Tokyo

关键词: amino acid sequence;    cyanobacterium;    principal σ;    factor;    purification;    RNA polymerase;    rpoD1 gene;    Synechococcus sp;   

DOI  :  10.2323/jgam.43.17

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

RNA polymerase was purified from the unicellular cyanobacterium, Synechococcus sp. strain PCC 7942, and found to be associated with a 52 kilodalton (kDa) polypeptide. The determined N-terminal sequence of the polypeptide was identical to the predicted amino-acid sequence of the rpoD1 gene product. Furthermore, the rpoD1 gene is suggested to be indispensable for viability by the inability to disrupt the gene. These results indicate that the rpoD1 gene product is the principal sigma factor of RNA polymerase.

【 授权许可】

Unknown