Elemental uptake via immersion: a mass-marking technique for the early life-history stages of ­cephalopods

Marine Ecology Progress Series

Jayson M. Semmens¹  Nicholas L. Payne¹  Bronwyn M. Gillanders¹ 

关键词: Enriched isotope;    Connectivity;    Otolith chemistry;    ICP-MS;    Cephalopods;    Otolith chemistry;    Markers;    Mn;    Otolith;    Statolith;    Barium;   

DOI  :  10.3354/meps09235

学科分类：海洋学与技术

来源: Inter-Research

【 摘 要 】

ABSTRACT: Despite the ecological and increasing commercial significance of cephalopods worldwide, little attention has been paid to developing techniques that investigate the movement of early life-history stages. We evaluated the potential of mass-marking giant Australian cuttlefish Sepia apama hatchlings, via immersion of eggs in water enriched in ¹³⁷Ba isotope. Eggs were immersed in several ¹³⁷Ba concentrations, for varying durations, and at different stages of development. Statoliths of hatchlings from each combination of ¹³⁷Ba concentration (0.3 and 1 µg l^–1) and immersion time (2, 5 and 8 d) produced lower mean ¹³⁸Ba:¹³⁷Ba values than control hatchlings, and mark success was generally high (70–100% of statoliths were unequivocally marked across all treatments). Development stage was an important factor, with statoliths successfully marked only for eggs immersed in ¹³⁷Ba at 10 wk of age. These results suggest that marking of statoliths via enriched isotope immersion offers considerable potential for understanding juvenile dispersal and connectivity of cephalopod populations in nature.

【 授权许可】

Unknown