Interpretation of fast repetition rate (FRR) fluorescence: signatures of phytoplankton community structure versus physiological state

Marine Ecology Progress Series

David J. Suggett¹  C. Mark Moore¹  Richard J. Geider¹  Anna E. Hickman¹ 

关键词: Fast repetition rate;    Fluorescence;    PSII;    Effective absorption cross section;    Photochemical efficiency;    Phytoplankton;    Nutrient stress;   

DOI  :  10.3354/meps07830

学科分类：海洋学与技术

来源: Inter-Research

【 摘 要 】

ABSTRACT: Introduction of active chlorophyll a fluorescence protocols, in particular fast repetition rate (FRR) fluorometry, to oceanography and limnology 15 yr ago has enabled rapid assessment of photosynthetic physiology in situ. The FRR protocol generates simultaneous measurements of Photosystem II (PSII) effective absorption cross sections (termed σ_PSII) and photochemical efficiency (termed F_v/F_m). Both F_v/F_m and σ_PSII measurements have been utilised to examine the effects of physiological stress on the photosynthetic apparatus of phytoplankton in an ever growing number of fluorescence-based studies. However, it is now becoming clearer that in situ values of F_v/F_m and σ_PSII also contain taxonomic information. Here, we present a synthesis of previously unpublished and published data, which show that F_v/F_m and σ_PSII vary principally with broad-scale changes in community structure. These patterns observed in situ conform to trends observed in laboratory-grown cultures of a range of phytoplankton taxa. The magnitudes of variability in F_v/F_m and σ_PSII driven by changes in phytoplankton community structure often exceed that induced by nutrient limitation (as determined from controlled nutrient addition experiments). An exception to this general trend occurs in high-nutrient, low-chlorophyll a (HNLC) regions, where strong phenotypic changes in F_v/F_m and σ_PSII have been repeatedly demonstrated on relief of iron limitation. Overall, FRR fluorescence measurements of both F_v/F_m and σ_PSII in natural populations represent a combination of the taxonomic ‘signature’ (values of F_v/F_m and σ_PSII determined by the taxa present) within the phytoplankton community that is further modified according to the (photo-) physiological status. As such, fluorescence-based investigations of mixed populations must account for potential variations in phytoplankton community structure before interpretations of physiological status are made.

【 授权许可】

Unknown