Phytoplankton class determination by microscopic and HPLC-CHEMTAX analyses in the southern Baltic Sea

Marine Ecology Progress Series

Jean-Fran#xe7ois Berthon¹  Elif Eker-Develi¹  Dirk van der Linde¹ 

关键词: CHEMTAX;    Pigments;    Phytoplankton carbon;    C:chl a;    Baltic Sea;   

DOI  :  10.3354/meps07319

学科分类：海洋学与技术

来源: Inter-Research

【 摘 要 】

ABSTRACT: The contribution of phytoplankton groups to total chlorophyll a (chl a) was derived using CHEMTAX from pigment measurements by HPLC and compared with the carbon (C) biomass estimations obtained from microscopy in the southern Baltic Sea in April 2005. Five different matrices of pigment:chl a input ratios, derived from the literature, were tested. Successive runs of CHEMTAX showed peridinin:chl a for dinoflagellates and fucoxanthin:chl a for diatoms to converge at 0.452 ± 0.02 (mean ± SD) and 0.489 ± 0.03, respectively, with initial ratios varying by a factor of 2 to 3 across matrices. The 2 techniques were in relatively good agreement for the dominant phytoplankton groups. Peridinin, diadinoxanthin, chlorophylls c₁ and c₂ (here grouped together as chl c₁+c₂), fucoxanthin and alloxanthin were the principal accessory pigments; dinoflagellates, diatoms and cryptophytes were the groups forming the majority of the C biomass. Diadinoxanthin and chl c₁+c₂ were mainly associated with the dominant dinoflagellates rather than with other phytoplankton classes. Excluding cyanophytes, the correlation between carbon biomass of other minor phytoplankton groups and their chl a was either poor or not significant due to uncertainties in either microscopic counts or CHEMTAX classification. There was a good correlation between carbon biomass of phytoplankton and chl a. The estimated C:chl a ratio of total phytoplankton varied between 8 and 40 (in average 20 ± 7), with a higher value for dinoflagellates (30 ± 17) than for diatoms (9 ± 7). Fucoxanthin-containing small flagellates might have led to the overestimation of the diatom contribution by CHEMTAX at a few stations.

【 授权许可】

Unknown