Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by Bacillus sp

Revista de Microbiologia

Gomes, Eleni¹  Soares, Márcia M.C.N.¹  Universidade Estadual Paulista, São José do Rio Preto, Brasil¹  Silva, Roberto da¹  Universidade Estadual Paulista, José do Rio Preto¹ 

关键词: : Bacillus sp;    polygalacturonase;    pectinolytic activity;   

DOI  :  10.1590/S0001-37141999000400002

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturonase production indicated that five strains of Bacillus sp produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0 – 7.0, optimum temperatures between 45oC and 55ºC, stability at temperatures above 40ºC and in neutral and alkaline pH, were determined.

【 授权许可】

Unknown