Bacteria isolated from a sugarcane agroecosystem: their potential production of polyhydroxyalcanoates and resistance to antibiotics

Revista de Microbiologia

Grisi, Breno M.¹  Universidade Federal da Paraíba, João Pessoa, Brasil¹  Lima, Teresa Cristina S. de¹  Universidade Federal da Paraíba¹  Bonato, M. Christina M.¹ 

关键词: : soil bacteria;    poly-b-hydroxyalkanoate;    PHA;    resistance to antibiotics;    sugarcane agroecosystem;    vinasse;   

DOI  :  10.1590/S0001-37141999000300006

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

In this investigation, a sugarcane agroecosystem at a coastal tableland, in the northeast of Brazil, was screened to obtain bacteria strains able to synthesize poly-b-hydroxyalkanoates (PHA), using sucrose as the main carbon source. The potential to synthesize PHA was tested qualitatively by Sudan Black staining of colonies growing in different carbon sources: sucrose, glucose, fructose, propionate and cellulose. In a typical sugarcane crop management system, the plantation is burned before harvesting and vinasse, a byproduct of alcohol production, is used in a fertirrigation system causing, probably, selective pressures on the microbiota of natural environments. Eightytwo bacteria strains, belonging to 16 different genera and 35 different species, were isolated. The data showed that 11 strains (ca 13%), nine of which belonging to the genus Pseudomonas, presented a strong Sudan Black staining in several carbon sources tested and, simultaneously, showed multiple resistance to antibiotics. Resistance to antibiotics is an advantageous feature for the biotechnological production of PHAs. The total number of isolates with multiple resistance to antibiotics was 73, and 38% of them belong to the genus Pseudomonas. Among the isolates, ca 86% and 43% grew in the presence of 10-100 U/ml of penicillin and/or 100-300 mg/ml of virginiamycin, respectively. These antibiotics are utilized in the alcohol distillery we investigated. The results suggest that some agroecosystem environments could be considered as habitats where bacteria are submitted to nutritional unbalanced conditions, resulting in strains with potential ability to produce PHAs, and also, to an increase in the microbial diversity.

【 授权许可】

Unknown