伊勢いもの組織培養における多芽体およびマイクロチューバーの形成に及ぼす植物成長調節物質, 光条件および培地の影響

日本作物学会紀事

今井 勝¹  酒井 隆成¹ 

[1] 明治大学農学部

关键词: Jasmonic acid;    ジャスモン酸;    Tuber;    ツクネイモ;    Dioscorea opposita;    Naphthalene acetic acid;    ナフタレン酢酸;    Benzyladenine;    ベンジルアデニン;    Yam;    ヤマノイモ;   

DOI  :  10.1626/jcs.77.481

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

We obtained the microtubers of Ise-imo from the multiple shoots formed by the tissue cultures of shoot-tip and node explants with solid or liquid MS medium. Shoot-tip explants cultured with benzyladenine (0.1—2 mg L-1 ; BA) and naphthalene acetic acid (0.1—0.5 mg L-1), or node explants cultured with BA (0.1—5 mg L-1) differentiated multiple shoots. Multiple shoots were maintained on a medium with 5 mg L-1 BA and their growth occurred on the subculture medium without plant growth regulators. Although microtubers were formed immediately after the addition of jasmonic acid (JA), they were formed without JA after a substantial time lag. More microtubers were formed in the dark than in the light but their enlargement was vice versa. Obtained microtubers (heavier than 120 mg FW) could be used successfully for the ordinary cultivation after storage in a refrigerator at 8°C for one month.

【 授权许可】

Unknown