Investigation of the Callus Induction Medium and the Regeneration Medium in Rice Anther Culture

Japanese Journal of Crop Science

Motoyasu OTANI¹  Takiko SHIMADA¹  Yoko IKUTA¹ 

关键词: Callus induction medium;    イネ葯培養;    Japonica rice;    カルス形成培地;    Regeneration medium;    再分化培地;    Rice anther culture;    日本稲;   

DOI  :  10.1626/jcs.68.151

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

We determined the callus induction medium and the regeneration medium to efficiently utilize the anther culture technique in rice breeding. The medium containing N6 basal medium supplemented with 4 mg L-1 of 2, 4-D effectively induced calli from microspores of some japonica varieties and two F1 hybrids with japonica varieties. The regenerated plants containing green and/or albino shoots were produced efficiently from microspore-derived calli on the regeneration medium containing LS basal medium supplemented with sorbitol (30000 mg L-1), CH (2000 mg L-1), MES (1000 mg L-1), NAA (2 mg L-1), and kinetin (0.5 mg L-1) solidified by gellun gum (4000 mg L-1).

【 授权许可】

Unknown