A Rapid and Convenient Method for a-Amylase Analysis of Wheat by an Auto-Analyzer and its Application to Estimation of Maximum Amylograph Viscosity

Japanese Journal of Crop Science

Satoshi NAKATSU¹  Tomoyuki NOMURA¹  Toru IMAI¹ 

关键词: α-Amylase activity;    アミログラム;    Auto-analyzer;    α-アミラーゼ活性;    Maximum amylograph viscosity;    オートアナライザー;    Pre-harvest sprouting;    小麦;    Wheat;    穂発芽;   

DOI  :  10.1626/jcs.66.35

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

In order to estimate the maximum amylograph viscosity of wheat, an automatic method for the measurement of α-amylase activity was developed using a commercial blocked ρ-nitrophenyl maltoheptaoside as a suitable substrate. A Type AAIIauto-analyzer (Bran + Luebbe) partially modified can rapidly and conveniently analyze α-amylase activity at almost 50 samples per hour, and can save the cost of reagents as compared to manual analysis. There was no significant difference between α-amylase activity measured by hand and the auto-analyzer. The α-amylase activity in flour correlated well with the maximum amylograph viscosity (r=-0.922). The correlation coefficient between α-amylase activity and maximum amylograph viscosity was slightly lower in ground wheat (r=-0.906). With regard to fresh wheat, there was no apparent relationship in wheat sampled before maturity, but a high correlation (r=-0.884) was exhibited after maturity. To monitor the levels of maximum amylograph viscosity in cargo samples and classify grades, we made tentative criterion for α-amylase activity in fresh wheat. Group 1: most of the wheat with α-amylase activity below 300 mU/g showed a high amylograph viscosity (mean=755B.U.). Group 2: wheat with α-amylase activity between 300 to 500 mU/g widely ranged from low to high viscosity (mean=502B.U.). Group 3: most of the wheat with α-amylase activity over 500 mU/g showed unacceptably levels of amylograph viscosity (mean=109B.U.). Therefore, the auto-analyzer can be utilized to rank wheat quality at receival points, and can also be adapted as a selection method for the development of low α-amylase varieties in wheat breeding.

【 授权许可】

Unknown