Fine Structures Concerning Storage Protein Accumulation in Developing Cotyledon of Broad Bean (Vicia faba L.)

Japanese Journal of Crop Science

Nobuo CHONAN¹  Toshiaki MATSUDA¹  Kouichi KASHIWABA¹ 

关键词: Broad bean;    液胞;    Cotyledon;    子葉柔細胞;    Protein accumulation;    粗面小胞体;    Protein body;    ソラマメ;    Rough endoplasmic reticulum;    タンパク顆粒;    Ultrastructure;    タンパク質蓄積;    Vacuole;    微細構造;   

DOI  :  10.1626/jcs.64.717

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Developing leguminous seeds are laid down the reserves accumulation in the parenchyma cells of the cotyledone. The sequence of protein accumulation of developing broad bean seeds has been followed by scanning and transmission electron microscopy. At the early maturation stage, protein was initially deposited in central vacuoles. The central vacuoles were rapidly subdivided to form many smaller protein-filled vacuoles. These vacuoles were surrounded by developed rough endoplasmic reticulum (RER). From the middle of maturation and on, the developed RER system was maintained in the cytoplasm. Transmission eletron microscopy revealed that vacuoles filled up with reserve protein were seen to be surrounded by increased RER and highly electron-dense vesicles. These vesicles increasd during protein accumulation and have some relation with protein transport. The terminal of RER becomes a dilated structure, in which protein bodies appear to be formed. However, this structure decreased at a later stage of maturation. The protein vesicles become the mature protein bodies at the last stage of maturation. Starch grain formation was rapidly proceeded in plastids at during the middle of maturation. It is suggested that protein accumulation of developing broad bean seeds is closely related with RER, and that protein bodies are formed by two pathways : subdivision from central vacuoles, and the protein deposition in the dilated RER.

【 授权许可】

Unknown