Ultrastructure of Electrofused Products from Pansy (Viola tricolor) Mesophyll and Wild Viola (V. patrinii) Petiole Callus Protoplasts

Japanese Journal of Crop Science

Eizo MAEDA¹  Tadahiko SATO¹  Takeshi TANIGUCHI¹  Chung Han CHUNG¹  0h Chang KWON¹ 

关键词: Electrofusion;    電気融合;    Pansy;    パンジー;    Protoplast;    微細構造;    Ultrastructure;    プロトプラスト;    Wild viola;    野生ビオラ;   

DOI  :  10.1626/jcs.61.469

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Ultrastructural observations were undertaken to study the viability of fusion products by electrofusing the protoplasts from the mesophyll cells of pansy leaves and the cultured callus cells of wild viola petioles. The result showed that the fusion product was formed between the two different protoplasts and two distinct regions were observed in its cytoplasm ; one region contained chloroplasts from pansy mesophyll and the other, plastids from wild viola callus. In other cases, however, the fusion product was seen which might be derived from merely the wild viola protoplasts because of the lack of well-developed chloroplasts. Also, other cellular components, such as nuclei, endoplasmic reticula, small vesicles and osmiophilic granules, were clearly found in the fusion products. Further, the fate of fused protoplasts is discussed on the basis of the ultrastructural features.

【 授权许可】

Unknown