Growth Regulating Action of Brassinolide on Plants : II. Effect of brassinolide on the translocation of assimilate in rice plants during the ripening stage

Japanese Journal of Crop Science

Hitoshi SAKA¹  Seiichi FUJII¹ 

关键词: Autoradiography;    イネ(Oryza sativa L.);    Brassinolide;    オートラジオグラフィー;    ^<14>C-tracer method;    ^<14>C-トレーサー法;    Rice (Oryza sativa L.);    転流;    Translocation;    ブラシノライド;   

DOI  :  10.1626/jcs.61.193

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

To determine the effect of brassinolide (BR, 2α, 3α, 22R, 23R-tetrahydroxy-24S-methyl-B-homo-7-oxa-5α-cholestan-6-one) on the translocation of assimilate during the ripening of rice plants (Oryza sativa L. cv. Nipponbare), tracer studies of 14C were carried out with pot-cultivated rice plants under greenhouse conditions. BR was sprayed twice with foliar application at concentrations of 10-3 and 10-2 ppm (30 ml/pot), respectively, 10 days before the heading and the heading stage. Rice plants were fed with 14CO2 to make them carry out 14CO2 assimilation at the flowering stage. Both the distribution and radioactivity of 14C in each plant organ were determined by two methods using autoradiography and a liquid scintillation spectrometer. Most of the 14C presented in the leaf blades at 1 hour after assimilation. The 14C in the leaf blades rapidly decreased after 1 day and subsequently translocated into the culms and panicles. Finally, most of the 14C translocated into the panicles after 21 days. Compared with the untreated controls, BR enhanced the incorporation of the 14C into the leaf blades after 1 hour, lowered the 14C in culms, and promoted the accumulation of the 14C in the panicles from after 3 days. These results suggest that the promotive effect of BR on the ripening of rice plants is attributed to the promotion of the translocation of photosynthates.

【 授权许可】

Unknown