Effect of Pre-treatment with Excess Sucrose or Mannitol on Plant Regeneration from Rice Callus Cultures

Japanese Journal of Crop Science

Eizo MAEDA¹  Nobuhiro HIGUCHI¹ 

关键词: Callus culture;    イネ;    Mannitol;    カルス培養;    Oryza sativa;    植物体再生;    Plant regeneration;    è”—ç³–;    Scanning electron microscopy;    走査電子顕微鏡;    Sucrose;    マニトール;    Water stress;    水ストレス;   

DOI  :  10.1626/jcs.60.122

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The formation of white and compact rice callus cultures was promoted by preculturing mature seed -derived celli on the medium containing a high concentration of sucrose. The obtained white and compact callus had a high regenerating ability on the regeneration medium containing 5 × 10-5M kinetin. When mannitol was added to the medium at the same osmotic level as excess sucrose treatment, it offered the intermediate effect between 3% (control) sucrose and 69L (high concentration) sucrose. Scanning electron microscopic examination revealed that the calli which retained the high regenerating ability had a tendency to show a smooth-surfaced appearance.

【 授权许可】

Unknown