Flash-Induced Absorption Change at 703 nm in Intact Leaves of Kidney Bean Plants

Japanese Journal of Crop Science

Hiromi KANO¹  Naoki KATSURA¹  Mika KOIZUMI¹  Katsumi INADA¹ 

关键词: Absorption change;    インゲンマメ;    Flash photolysis;    吸光度変化;    Intact leaf;    生葉;    Kidney bean;    フラッシュ・フォトリシス;    P700;   

DOI  :  10.1626/jcs.57.332

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

The absorption change at 703 nm induced by Hash actinic light in intact leaves of kidney bean plants was measured. The dependency of the absorption change upon the intensity of the detecting light and intensity and wavelength of actinic flash was examined in order to investigate photosystems and electron transport system in vivo. The light saturation of P700 oxidation and reduction by flash light was represented by hyperbolic curves, while that of P700 oxidation by detecting light was represented by an exponential curve. The action spectra of P700 oxidation and reduction at shorter wavelengths than 600 nm fluctuated in almost the same pattern, showing a minimum at 481 nm and a peak around 450 nm. Apparent differences between the two were observed above 630 nm. The action spectrum of P700 oxidation showed a peak at 621 nm, decreased at 683 nm and showed another peak at 700 nm, while the spectrum of P700 reduction decreased above a wavelength of 660 nm. Based on the analysis by the present method, the photoreactions observed in leaves with dense pigments were discussed.

【 授权许可】

Unknown