Studies on Morphology and Cytochemistry in Blood Cells of AyuPlecoglossus altivelis altivelis

Journal of Veterinary Medical Science

Hiroyuki HORIUCHI¹  Kojin NAKADA¹  Shuichi FURUSAWA¹  Kuniyasu FUJISAWA² 

[1] Department of Molecular and Applied Bioscience Graduate School of Biosphere Sciences, Hiroshima University, 1�?4�?4 Kagamiyama, Higashi-hiroshima, Hiroshima 739�?8528, Japan;Takahashigawa Fisheries Cooperative Association, 52 Teppouchou, Takahashi, Okayama 716�?1146, Japan

关键词: ayu;    characterization;    classification;    Percoll;    peripheral blood;   

DOI  :  10.1292/jvms.13-0584

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(1)Peripheral blood cells from ayu, Plecoglossus altivelisaltivelis, were separated using a density gradient. Blood cells were thensmeared using Shandon Cytospin and subjected to cytochemical staining. Blood cells werecategorized based on morphological and cytochemical characteristics, and the densityfractionation range and nucleus area/cell area ratio were observed. Lymphocytes aredistinguished from neutrophils by their basophilic cytoplasm and Golgi-like field. Thefeatures of chromatin in thrombocytes are different from those of lymphocytes orneutrophils, but some small neutrophils have similar chromatin. Therefore, it is necessaryto perform peroxidase staining to distinguish small neutrophils from thrombocytes.Basophils have large basophilic granules in cytoplasm. Based on density fractionation ofblood cells, thrombocytes in the low-density area were separated from other blood cells.Identification of peripheral blood cells from ayu was possible with these stainingmethods. Monocytes/macrophages from spleen are specifically positive for esterase stainingby α-naphthyl butyrate. As a result, thrombocytes, lymphocytes, neutrophils, basophils andmonocytes/macrophages were identified in smears from peripheral blood or spleen tissue. Inthis paper, we confirmed that the peripheral blood corpuscles of ayu are able to beidentified using the present staining methods.

【 授权许可】

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