Retrospective Analysis of Sheep Scrapie by Western Blotting with Formalin-Fixed Paraffin-Embedded (FFPE) Tissues

Journal of Veterinary Medical Science

Kumiko KIMURA¹  Kentaro MASUJIN²  Shirou MOHRI²  Kohtaro MIYAZAWA²  Gantsetseg DORJ²  Takashi YOKOYAMA²  Hiroyuki OKADA² 

[1] Subtropical Disease Research Division, National Institute of Animal Health, Chuzan, Kagoshima 891-0105, Japan;Prion Disease Research Center, National Institute of Animal Health, Tsukuba 305-0856, Japan

关键词: FFPE;    prion;    PrPSc;    scrapie;    WB;   

DOI  :  10.1292/jvms.12-0037

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)An abnormal isoform of prion protein (PrPSc) was extracted from formalin-fixed paraffin-embedded (FFPE) tissues from sheep and analyzed by western blotting. PrPSc immunoreactivity against anti-PrP monoclonal antibody T2, which recognizes discontinuous PrP sequences, differed amongst individual scrapie sheep cases. This may reflect structural differences in PrPSc that have been formalin-fixed prior to their extraction. This study indicates that western blotting by using FFPE tissues is useful for the retrospective analysis of transmissible spongiform encephalopathies in which only formalin-fixed samples are available and in conducting transmissible spongiform encephalopathies surveillance where freezing system is insufficient.

【 授权许可】

Unknown