Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil

Ciência Rural

Muniz, Marlove Fátima Brião²  Blume, Elena²  Vaz, Marcos André Braz¹  Salgado-Neto, Geraldo²  Guedes, Jerson Vanderlei Carús² 

[1] Universidade Federal de Santa, Santa Maria, Brazil;Universidade Federal de Santa Maria, Santa Maria, Brazil

关键词: Biological control;    dissemination;    Fusarium oxysporum;    interactions;    Melolonthidae;    white grubs;   

DOI  :  10.1590/0103-8478cr20150471

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagates andDiloboderus abderus (Coleoptera: Melolonthidae ) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae . Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporum by Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus (Coleoptera: Melolonthidae ) under soybean in Brazil.

【 授权许可】

Unknown