Efficacy of common disinfectants and terbinafine in inactivating the growth of Batrachochytrium dendrobatidis in culture

Diseases of Aquatic Organisms

Porsha D. Reed¹  Marcy J. Souza¹  Debra L. Miller¹  David A. Bemis¹  Kienan K. Gold¹  Matthew J. Gray¹ 

关键词: Chytrid fungus;    Amphibian;    Chlorhexidine;    Sodium hypochlorite;    Pro-San;    Virkon;    Ranaviruses;   

DOI  :  10.3354/dao02670

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Use of disinfectants by biologists, veterinarians, and zoological facilities is a standard biosecurity procedure to prevent contamination and the spread of pathogens. We tested the efficacy of 5 disinfectants and 1 anti-fungal treatment, at 1 and 5 min contact durations, in inactivating Batrachochytrium dendrobatidis (Bd) grown on tryptone media. Our study focused on concentrations of disinfectants known to inactivate ranaviruses, which can be found at the same sites as Bd and can concurrently infect amphibians. Disinfectants tested were chlorhexidine gluconate (0.25, 0.75, and 2%), Pro-San (0.19, 0.35, and 0.47%), Virkon S (1%), household bleach (0.2, 1, and 3%), and Xtreme Mic (5%). The anti-fungal was terbinafine HCl at 0.005, 0.05, 0.1, and 1 mg ml^–1. Inactivation of Bd was determined by microscopic evaluation of zoospore motility and growth of colony mass after 14 d. All disinfectants were effective at inactivating zoospore motility and colony growth of Bd at all concentrations and both contact times; however, terbinafine HCl inactivated Bd at only the highest concentration tested (1 mg ml^–1) and 5 min duration. Thus, a minimum of 0.25% chlorhexidine gluconate, 0.19% Pro-San, 1% Virkon, 0.2% bleach, and 5% Xtreme Mic with 1 min contact was sufficient to inactivate Bd. Also, terbinafine HCl (1 mg ml^–1) with a 5 min contact time might be effective in treating amphibians infected with Bd. Based on this study and previously published findings, 0.75% Nolvasan, 1% Virkon S, and 3% bleach with 1 min contact are sufficient to inactivate both Bd and ranaviruses.

【 授权许可】

Unknown