Role of CXCR4 and SDF-1 in Mammary Tumor Metastasis in the Cat

Journal of Veterinary Medical Science

Bunei SYUTO²  Masami MORIMATSU²  Takayuki NAKAGAWA⁴  Nobutaka FUJII¹  Toshina OONUMA²  Hirokazu TAMAMURA¹  Shizu HASHIMOTO³  Nobuo SASAKI⁴  Rina UYAMA⁴  Hozumi YAMAMURA³  Munekazu NAKAICHI⁵ 

[1] Graduate School of Pharmaceutical Sciences, Kyoto University;Laboratory of Veterinary Physiology, Faculty of Agriculture, Iwate University;Kitagawa Animal Hospital;Laboratory of Veterinary Surgery, Division of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University

关键词: CXCR4;    feline;    mammary tumor;    metastasis;    SDF-1;   

DOI  :  10.1292/jvms.65.1069

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(5)It has recently been suggested that the chemokine receptor CXCR4 and its ligand SDF-1 (CXCL12) promote metastasis of various cancers in humans. Since feline mammary tumors also metastasize to distant organs frequently, we used real-time quantitative PCR to examine the expression of feline CXCR4 (fCXCR4) in ten feline mammary tumor cell lines and seven feline mammary tumor tissues, and also the expression of feline SDF-1 (fSDF-1) in various organs. Cell lines derived from metastatic regions expressed more fCXCR4 than those derived from primary tumors. Mammary tumor tissues overexpressed more fCXCR4 than normal mammary tissues. Organs with high levels of fSDF-1 expression represent common sites of metastasis. Migration assays using the feline mammary tumor cell line NAC were also performed to test the activity of TN14003 and TC14012, antagonists of human CXCR4, to antagonize fCXCR4 expressed on NAC cells. TN14003 and TC14012 inhibited migration of NAC cells. We conclude that fCXCR4 may be a therapeutic target for feline mammary tumors.

【 授权许可】

Unknown