Proliferation and osteoblastic differentiation of bone marrow stem cells: comparison of vertebral body and iliac crest

European Spine Journal

Eui Kyun Park¹  Byung-Chul Park⁴  Woo-Kie Min⁵  Min-Jung Son⁵  In-Ho Jeon⁵  Shin-Yoon Kim⁵  Hee Kyung Jin²  Jae-Sung Bae³ 

[1] Department of Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu, Korea;Department of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea;Department of Physiology, School of Medicine, Kyungpook National University, Daegu, Korea;Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, 50 Samduk 2-ga, Jung-gu, Daegu, 700-712 Korea;Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Daegu, Korea

关键词: Vertebral body;    Iliac crest;    Bone marrow stem cells;    Proliferation;    Osteoblastic differentiation;   

DOI  :  10.1007/s00586-010-1424-8

学科分类：骨科学

来源: Springer

【 摘 要 】

Bone marrow stem cells (BMSCs) can be obtained from the vertebral body (VB) and iliac crest (IC) for augmenting spinal arthrodesis. However, it is still not evaluated, which of the two sites would have a better BMSCs potential on Proliferation and osteoblastic differentiation is still not evaluated. Fourteen patients (10 men and 4 women) undergoing posterolateral lumbar arthrodesis and pedicle screw instrumentation were involved. The mean age was 54.7 years (range 31–75 years). Bone marrow aspirates were obtained from the vertebral body through the bilateral pedicle and were quantified relative to matched, bilateral aspirates from the iliac crest that were obtained from the same patient and at the same time. The mononuclear cell count and concentration of BMSCs were calculated and compared. Proliferation and osteoblastic differentiation of each of the BMSCs were characterized using biochemical and molecular biology techniques. Concentration (cells/mL) of BMSCs from VB and IC were 3.73 × 103 and 3.19 × 103, respectively (P > 0.05). VB and IC exhibited similar proliferation pattern at 3, 5 and 7 days, but BMSCs from the VB exhibited an increased mineralization staining with Alizarin Red S at 14 days. BMSCs from both anatomic sites expressed comparable levels of CD29, CD34, CD44, CD90 and CD105. VB and IC displayed similar levels of expression of ALP, type I collagen and osterix, but VB expressed higher level of osteocalcin and Runx-2, especially at 14 and 21 days. Our studies show that BMSCs from VB have osteogenic differentiation potential similar to IC. Based on these findings, we suggest that BMSCs from VB would be comparable candidates for osseous graft supplementation especially in spinal fusion procedures.

【 授权许可】

Unknown