Viral replication and shedding during acute infection and after dexamethasone induced reativation of latency in calves inoculated with bovine herpesvirus type 1 (BHV-1) and 5 (BHV-5)

Ciência Rural

Vogel, Fernanda Silveira Flores¹  Weiblen, Rudi²  Arenhart, Sandra³  Winkelmann, Evandro Reinoldo³  Lima, Marcelo de³  Flores, Eduardo Furtado²  Mayer, Sandra Vanderli²  Mazzutti, Ketty Cristina³ 

[1] Universidade Federal de Santa Maria, Santa Maria;Universidade Federal de Santa Maria

关键词: bovine herpesvirus type 1;    BHV-1;    BHV-5;    dexamethasone;    latent infection;   

DOI  :  10.1590/S0103-84782004000500047

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The efficiency of the establishment and reactivation of latent infection by bovine herpesviruses types 1 and 5 (BHV-1 and 5) was compared. Calves inoculated intranasally with BHV-1 (SV-265, n=6) or BHV-5 (SV-507, n=6) presented a mild to moderate nasal discharge and shed virus in nasal secretions in titers up to 107.81TCID50/ml (mean tissue culture infectious dose) during an average of 10.5 days (6-15 [BHV-1]) or up to 106.7 TCID50/ml during 15.3 days (13-18 [BHV-5]). Dexamethasone administration (Dx; 0.5mg/kg) at day 60pi resulted in reactivation of the infection in all calves. Virus shedding in nasal secretions was detected in titers up to 105.5TCID50/ml during 6 to 9 days (mean: 7.8) in calves inoculated with BHV-1 and in titers up to 106.1TCID50/ml (3 to 12 days, mean: 7.5) in calves inoculated with BHV-5. These results showed that BHV-1 and BHV-5 were capable of establishing and reactivating the latent infection at comparable levels.

【 授权许可】

CC BY-NC