Methods to enhance the intensity of intranuclear bacilliform virus infection in Cherax quadricarinatus

Diseases of Aquatic Organisms

Bradford Cullen¹  Kerry Claydon¹  Leigh Owens¹ 

关键词: Cherax quadricarinatus;    Intranuclear bacilliform virus;    Histology;   

DOI  :  10.3354/dao060173

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Many studies have examined the morphology, pathology and epizootiology of the intranuclear bacilliform virus (IBV) of Cherax quadricarinatus, but little research has been conducted to acquire specific knowledge of the virus. This ispartly due to difficulties in detecting the virus and in obtaining sufficient material for viral isolation and purification. As quantified by light microscopy, we significantly (p < 0.01) enhanced IBV intensities from 10.56 to 16.67% in C.quadricarinatus by using salinity stress (12 ppt) and ingestion of infected hepatopancreatic tissue, which increased intensities from 4.33 to 10.77%. It was also found that phosphotungstic acid-eosin stain was superior to standard haematoxylin andeosin stain in visualizing IBV inclusion bodies. It is expected that these new techniques will enhance the detectability of the virus and provide sufficient viral material for viral purification, characterization and development of molecular tools fordetection and phylogenetic analysis.

【 授权许可】

Unknown