The Efficiency of Trichoderma harzianum and Aneurinobacillus migulanus in the Control of Gladiolus Corm Rot in Soil-Less Culture System | Science Publications

American Journal of Agricultural and Biological Sciences

Nosir Walid¹  McDonald Jim¹  Woodward Steve¹ 

关键词: Gladiolus;    Trichoderma harzianum;    A. migulanus;    F. oxsporum f. sp. gladioli soilless culture;    CFU;   

DOI  :  10.3844/ajabssp.2010.436.445

学科分类：农业科学（综合）

来源: Science Publications

【 摘 要 】

Problem statement: Gladiolus is cultivated commonly for cut flower production, frequentlyas a protected crop. The glasshouse or polytunnel environment, providing excellent conditions for flowerproduction, also make conditions more favorable for disease to develop. Approach: The pathogenFusarium oxysporum f. sp. gladioli causes wilt diseases in a wide range of economically important plantsand can have devastating effects on crop production. Trichoderma harzianum and Aneurinobacillusmigulanus were tested separately and in combination for controlling F. oxysporum f. sp. gladioli activityin soilless culture using Perlite as the substrate. Results: The efficiency of both of antagonists againstcorm rot was evaluated based on vegetative and root growth parameters and on flowering parameters. T.harzianum was more effective than A. migulanus in disease suppression and also enhanced plantgrowth, increased flower production and quality. A. migulanus enhanced plant growth when tested alone.The mixture of antagonists reduced the efficiency of T. harzianum. Numbers of T. harzianum CFU in thesubstrate and on corms increased following application compared with treating with both antagonists. NoT. harzianum was detected in the substrate by 120 day after planting, however, A. migulanus CFUsignificantly decreased on corms when inoculated in combination with T. harzianum and F. oxysporumf. sp. gladioli. However, A. migulanus CFU was not detected in the substrate of the same combination.SEM and Glasshouse results suggested that suppressive mechanisms of T. harzianum and A. migulanusdiffered. T. harzianum appeared to operate through a combination of antibiosis and substrate competition,whereas A. migulanus produced an electron-dense substance which may have inhibited the penetration ofhost tissues by F. oxysporum f. sp. gladiolus. Greater growth of T. harzianum was observed wheninoculated alone or with F. oxysporum f. sp. gladioli hyphae. Conclusion: It was concluded that T.harzianum provided a more efficient and effective control of F. oxysporum f. sp. gladioli corm rot ofGladiolus when inoculated without A. migulanus.

【 授权许可】

Unknown