Propagação in vitro de sacaca (Croton cajucara Benth.): entendimentos sobre a dificuldade no desenvolvimento de protocolos de micropropagação da espécie

Biotemas

Jonny Everson Scherwinski-Pereira²  Tatiane Loureiro da Silva¹  Maria Aparecida Alves Pereira³ 

[1] Instituto Federal de Educação, Ciência e Tecnologia do Acre �? IFAC, Campus de Xapuri, Rua Coronel Brandão, nº 1622, CEP: 69.930-000, Xapuri �? AC.;Embrapa Recursos Genéticos e Biotecnologia, Av. W5 Norte (final), Parque Estação Biológica, CEP 70770-917, Brasília - DF;Engenheira agrônoma, Universidade Federal do Acre, Centro de Ciências Biológicas e da Natureza Campus Universitário, BR-364, Km 04, CEP: 69.920-900, Rio Branco �? AC.

关键词: Amazônia;    Croton spp.;    Contaminação microbiana;    Linalol;    Plantas medicinais;    Organogênese;   

DOI  :  

学科分类：生物科学（综合）

来源: Universidade Federal de Santa Catarina * Centro de Ciencias Biologicas

【 摘 要 】

http://dx.doi.org/10.5007/2175-7925.2015v28n4p41Sacaca is a medicinal plant from the Amazonian biome and it has been regarded as a substitute for rosewood ( Aniba roseaodora ) to produce linalool. This paper aimed to evaluatein vitrovegetative propagation of sacaca, including the establishment of propagules from the field, decontamination protocols, and determination of multiplication rates, besides describing limiting aspects for the culture duringin vitroexperiments. We used 1.0 cm microcuttings with an axillary bud, collected from adult plants in the field. Disinfestation treatments were tested in the establishment, and there is an evaluation of the collecting month influence on the contamination rates. After disinfestation, microcuttings were placed in test tubes containing MS medium, added with BAP (0, 1, 2 and 3 mg L -1 ) and GA 3(0 and 0.5 mg L -1 ).In vitroestablishment of sacaca with 41.9% of sprouted microcuttings was obtained. The contamination rate reached 58.1% (65.4% caused by fungi and 34.6% by bacteria), with greater occurrence when propagules were collected between October and January, the rainiest months in the Amazon region. The increased BAP and GA 3concentrations in the culture medium provided significant improvements in the material multiplication rates. In spite of the results obtained, the species shows peculiarities and limitations toin vitrocultivation that were identified and described in this paper.

【 授权许可】

Unknown