Cloning and sequencing of envelope proteins (VP19, VP28) and nucleocapsid proteins (VP15, VP35) of a white spot syndrome virus isolatefrom Korean shrimp

Diseases of Aquatic Organisms

Sun A Cho¹  Jae Hak Park¹  Seung Hyeok Seok¹  Jong Hwan Park¹  Min Won Baek¹  Dong Jae Kim¹  Hui Young Lee¹ 

关键词: White spot syndrome virus;    WSSV;    Envelope proteins;    VP19;    VP28;    Nucleocapsid proteins;    VP15;    VP35;   

DOI  :  10.3354/dao060085

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Since our first report in 1998, white spot syndrome virus (WSSV) has become widespread on the southern and western coasts of Korea. Almost all shrimp in ponds die within 3 to 4 d after the first dead shrimp are observed with gross lesionsranging from abnormal red body discoloration to white spots in the cuticle. From one isolate, we cloned and sequenced WSSV genomic DNA coding for VP19 and VP28 envelope proteins and VP15 and VP35 nucleocapsid proteins. Putative protein sequences weresubmitted to GenBank and assigned accession numbers AY316119 (VP19), AY324881 (VP28), AY374120 (VP15) and AY325896 (VP35). At the nucleotide level, VP19, VP28 and VP15 sequences were, respectively,99, 100 and 100% identical to those of China, Indonesia,Japan and the United States and the VP35 sequence was 100% identical to that of a Taiwanese isolate. The deduced amino-acid sequences were 99 to 100% identical to those from other countries. In VP19, C and T in the foreign isolates were replaced by T andA in the Korean isolate at Positions 57 and 218 nt, respectively, downstream of A (+) of the VP19 start codon. The change at Position 218 nt resulted in valine in the foreign isolates being replaced by aspartate in the Korean isolate.

【 授权许可】

Unknown