Generation of Canine Dendritic Cells from Peripheral Blood Mononuclear Cells

Journal of Veterinary Medical Science

Tadaaki KUDO²  Toshiroh IWASAKI¹  Yasuyuki MOMOI¹  Natsuko TAGA²  Kazuaki YAMAZOE²  Hiroko YOSHIDA²  Kaori IDE¹ 

[1] Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Department of Veterinary Surgery, Faculty of Agriculture, Gifu University

关键词: canine;    dendritic cell;    immunotherapy;    PBMC;    phytohemagglutinin;   

DOI  :  10.1292/jvms.65.663

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Cited-By(13)Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 × 106 of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs.

【 授权许可】

Unknown