Cleavability of Hemagglutinin from an Extremely Virulent Strain of Avian Influenza Virus Containing a Unique Cleavage Site Sequence

Journal of Veterinary Medical Science

Dennis J. ALEXANDER¹  Taisuke HORIMOTO¹  Yoshihiro KAWAOKA¹  Toshihiro ITO¹ 

关键词: avian influenza virus;    cleavability;    hemagglutinin;   

DOI  :  10.1292/jvms.57.927

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(29)Cited-By(17)An avian influenza virus, A/turkey/England/50-92/91 (H5N1), showed extremely high virulence in chickens, although its hemagglutinin (HA) cleavate site sequence (R-K-R-K-T-R), having a nonbasic (Thr) residue at the second position (P-2) from the carboxyl terminus of HA1, does not conform to the previously established concensus sequence motif, X-X-R/K-X-R/K-R (X=nonbasic residue), for highly virulent phenotype of the H5 virus. When we evaluated the HA cleavability of this strain in chicken embryo fibroblast culture, we observed that, unlike other HAs with a Thr residue at P-2, this HA was efficiently cleaved. These findings suggest that a nonbasic residue at the P-2 does not affect its recognition and catalyzation by cleavage enzymes that are otherwise influenced by steric structure around the cleavage site.

【 授权许可】

Unknown