Relationship between Neubauer counting chamber and spectrophotometer used for the sperm concentration determination in collared peccaries (Pecari tajacu)

Ciência Rural

Castelo, Thiberio de Souza¹  Bezerra, José Artur Brilhante¹  Peixoto, Gislayne Christianne Xavier¹  Santos, Erika Aparecida Araújo¹  Silva, Andréia Maria da¹  Silva, Alexandre Rodrigues¹ 

[1] Universidade Federal Rural do Semi-Árido, Mossoró, Brasil

关键词: spectrophotometer;    collared peccary;    sperm concentration;    Tayassu tajacu;   

DOI  :  10.1590/0103-8478cr20130928

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of 283.9±30.8x106sperm m-1, varying from 30 to 640x106sperm mL-1. Values for tramitance varied from 36.9 to 96.3, under different wavelengths. No significant relationship was verified between two methods (P>0.05). Thus, the spectrophotometer is not recommended for routine procedures of sperm concentration measurement in collared peccaries.

【 授权许可】

Unknown