Synthetic circuit of inositol phosphorylceramide synthase in Leishmania: a chemical biology approach

Journal of Chemical Biology

Shailza Singh¹  Vineetha Mandlik¹  Dixita Limbachiya¹  Sonali Shinde¹  Milsee Mol¹ 

[1]National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007 India

关键词: IPCS;    Leishmania;    Genetic circuit;    Simulation;    Degradation rate;    Logic circuit;   

DOI  :  10.1007/s12154-012-0089-7

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge, there are no literature reports for the synthetic circuit construction for protozoan parasites. This paper describes the construction of genetic circuit for the targeted enzyme inositol phosphorylceramide synthase belonging to the protozoan parasite Leishmania. To explore the dynamic nature of the circuit designed, simulation was done followed by circuit validation by qualitative and quantitative approaches. The genetic circuit designed for inositol phosphorylceramide synthase (Biomodels Database—MODEL1208030000) shows responsiveness, oscillatory and bistable behavior, together with intrinsic robustness.

【 授权许可】

Unknown