Effect of Addition of Orvus ES Paste to Frozen Canine Semen Extender on Sperm Acrosomes

Journal of Veterinary Medical Science

Tatsuya HORI¹  Keiichi NAGAKUBO¹  Toshihiko TSUTSUI¹  Eiichi KAWAKAMI¹  Kaoru KOMORIYA¹  Masayoshi HASE¹  Noriko SHIMIZU¹ 

[1] Department of Reproduction, Nippon Veterinary and Animal Science University, 7-1 Kyonan-cho, 1 chome, Musashino-shi, Tokyo 180- 8602, Japan

关键词: acrosome;    canine;    Orvus ES Paste;   

DOI  :  10.1292/jvms.62.537

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(9)Cited-By(15)Using the triple-stain technique, we investigated whether sperm acrosomes in frozen canine semen were protected during freezing and thawing by addition of a surfactant, Orvus ES Paste (OEP), to the extender. Acrosomes were clearly shown to be protected by the addition of OEP to the entender when compared with those in sperm frozen without OEP addition (p<0.05).

【 授权许可】

Unknown