Effects of 17β-Estradiol, Bisphenol A and Tributyltin Chloride on Germ Cells of Caenorhabditis elegans

Journal of Veterinary Medical Science

Hidenobu HOSHI¹  Takashi UEMURA¹  Yoichi KAMATA¹ 

[1] Department of Veterinary Public Health, Division of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

关键词: bioassay;    Caenorhabditis elegans;    environmental pollutant;    germ cell;    reproductive toxicity;   

DOI  :  10.1292/jvms.65.881

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(37)Cited-By(19)Effects of a one-generation exposure to a natural estrogen, 17β-estradiol (E2), and environmental pollutants such as bisphenol A (BPA) and tributyltin chloride (TBTCL) on the number of germ cells were investigated in the hermaphrodite Caenorhabditis elegans. The eggs of gravid adult worms isolated by alkaline hypochlorite treatment were seeded on a test chemical-containing NGM (nematode growth medium) agar plate without cholesterol. After incubation for 6 days at 16°C, the germ cells of adult worms were stained with 4', 6-diamino-2-phenylindole dihydrochloride (DAPI). The staining procedure was completed within one hour and the stained germ cells were counted under a fluorescence microscope without dissection. The number of germ cells in the worms treated with E2 (10-10-10-6 M) and BPA (10-9-10 -5 M) was significantly increased. Maximal increases were observed at 10-8 M E2 (156 ± 15.3% of control) and 10-5 M BPA (168 ± 20.0 % of control). TBTCL (10-9-10-6 M) significantly decreased the number of germ cells. The minimal decrease was observed at 10-6 M TBTCL (30.2 ± 3.51% of control). These results indicate that changes in the number of germ cells are a sensitive indicator of the effects of chemicals on the reproductive system. Since the method described in this paper is a novel, simple, time- and money-saving bioassay, C. elegans is an excellent model with which to determine the reproductive toxicity of chemicals including environmental pollutants.

【 授权许可】

Unknown