Rutina aumenta a sobrevivência das células-tronco da crista neural e atua contra danos causados pela aflatoxina B1

Biotemas

Andrea Trentin¹  Jader Nones¹  Janaína Nones¹ 

[1] Universidade Federal de Santa Catarina

关键词: Aflatoxina B1;    Crista neural;    Diferenciação;    Proliferação;    Rutina;    Viabilidade celular;   

DOI  :  

学科分类：生物科学（综合）

来源: Universidade Federal de Santa Catarina * Centro de Ciencias Biologicas

【 摘 要 】

http://dx.doi.org/10.5007/2175-7925.2015v28n4p1 The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potential. The derivatives of the NC at the trunk level include neurons and glial cells of the peripheral nervous system, melanocytes, smooth muscle cells and some endocrine cells. The present work investigated, for the first time, the influence of aflatoxin B1 (AFB1) and the flavonoid rutin on the survival and proliferation of NC and NC-derived melanocytes. Quail NC cell cultures were treated with AFB1 (30 μM) and/or rutin (20 μM) for 6 days. Cell viability was assessed by MTT and trypan blue analyses and cell proliferation by BrdU staining. Melanocytes were identified by immunocytochemistry against the melanocyte-specific cellular marker MelEM. The AFB1 treatment decreased both NC cell viability and proliferation. The total number of MelEM-positive cells was also reduced after this treatment, an effect partially prevented by the addition of rutin. On the other hand, rutin added alone did not influence the NC cell population. Our results demonstrated that rutin increases the survival of the NC after damage caused by AFB1. However, additional studies are needed to better understand the mechanisms involved in AFB1 and rutin interactions.

【 授权许可】

Unknown