Analysis of the Epitopes on Staphylococcal Enterotoxin A Responsible for Emetic Activity

Journal of Veterinary Medical Science

Akio NAKANE²  Dong-Liang HU¹  Katsuhiko ONO¹  Shunji SUGII³  Mona Hamed Husien SALEH¹  Katsuhiko OMOE¹  Kunihiro SHINAGAWA¹ 

[1] )Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University;Department of Biotechnology, Hiroshima University School of Medicine;Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

关键词: emetic activity;    epitope;    staphylococcal enterotoxin;    Suncus murinus;    synthetic peptide;   

DOI  :  10.1292/jvms.63.237

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(8)To identify which region of staphylococcal enterotoxin A (SEA) is responsible for the emetic activity, twelve synthetic peptides corresponding to the entire SEA amino acid sequence and their respective anti-peptide antibodies were prepared and tested. The anti-peptide antibodies were tested for neutralization of SEA-induced emesis in Suncus murinus (Shrew mouse). The results indicate that SEA-induced emesis was neutralized by the mixture of three anti-peptide antibodies to A-7 (corresponding to amino acid residues 121-140), A-8 (141-160) and A-9 (160-180). These findings suggest that the regions corresponding to residues 121-180 may be the epitopes responsible for the emetic activity of SEA.

【 授权许可】

Unknown