Susceptibility of juvenile humpback grouper Cromileptes altivelis to grouper sleepydisease iridovirus (GSDIV)

Diseases of Aquatic Organisms

Asami Yamamoto¹  Ketut Mahardika¹  Zafran¹  Teruo Miyazaki¹ 

关键词: Humpback grouper;    Cromileptes altivelis;    Grouper sleepy disease iridovirus;    Tropical iridovirus;    Inclusion body-bearing cells;    Interferon-like effect;   

DOI  :  10.3354/dao059001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Susceptibility of juvenile humpback grouper Cromileptes altivelis to the grouper sleepy disease iridovirus (GSDIV) was examined. GSDIV-containing inocula for challenge were obtained using a filtrate of spleen tissues from donor fish(orange-spotted grouper Epinephelus coioides) infected with GSDIV. Groups injected with the primary filtrate showed lower mortalities (30 to 60%) than groups receiving the 10^-4 diluted inoculum (90 to 100% mortality). This result wascontrary to the expectation that fish challenged with a higher concentration of virus would show higher mortality. Electron microscopy revealed that moribund fish receiving the 10^-4 diluted inoculum displayed massive formation of typicalinclusion body-bearing cells (IBCs) containing an intracytoplasmic inclusion body with many virions in the 180-200 nm size range propagated within a virus assembly site. In contrast, survivors in fish receiving the primary filtrate showed the formation ofunusual IBCs containing an abnormal inclusion body that was characterized by the assembly of a small number of deformed virions. This impaired virus assembly appeared to prevent mortality in the challenged fish and was assumed to be due to aninterferon-like effect of a previously unknown substance that was passed on to the challenged fish with the tissue filtrate from the donor fish.

【 授权许可】

Unknown