Validation of the multiplex PCR for identification ofBrucella spp.

Ciência Rural

Almeida, Iassudara Garcia de¹  Orzil, Lívia de Lima¹  Soares Filho, Paulo Martins¹  Fonseca Júnior, Antônio Augusto¹  Preis, Ingred Sales¹  Jacinto, Fabrício Barcelos¹  Souza, Patrícia Gomes de¹ 

[1] Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, Brazil

关键词:  Brucellosis;    molecular identification;    zoonosis;    Brucella;    multiplex PCR RESUMO: Validou-se neste trabalho uma técnica de PCR Multiplex (mPCR) para detecção de Brucellaspp;   

DOI  :  10.1590/0103-8478cr20150065

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:A multiplex PCR technique for detection ofBrucellaspp. in samples of bacterial suspension was validated as a complementary tool in the diagnosis of the disease. This technique allows the characterization of the agent without performing biochemical tests, which greatly reduces the time for a final diagnosis, and provides more security for the analyst by reducing the time of exposure to microorganisms. The validation was performed in accordance with the Manual of Diagnostic Tests from OIE (2008) and following the requirements present in the ABNT NBR ISO/IEC 17025:2005. The mPCR validated in this study identified the different species of Brucella( Brucella abortus ,B. suis , B. ovise B. melitensis ) of bacterial suspension obtained from the slaughterhouse samples, as well as distinguished the biovars (1, 2 e 4; 3b, 5, 6 e 9) of B. abortusin grouped form and differentiated the field strains from vaccine strains, as a quick, useful and less expensive technique in diagnosis of brucellosis in Brazil.

【 授权许可】

Unknown