The requirement of environmental acidification for Ibaraki virus infection tohost cells

Journal of Veterinary Medical Science

Shusaku T. SHIBUTANI¹  Rie WATANABE¹  Hiroyuki IWATA¹  Yuya TSURUTA¹ 

[1] Laboratory of Veterinary Hygiene, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677�?1 Yoshida, Yamaguchi 753�?0841, Japan

关键词: acidic environment;    endocytosis;    IBAV infection;   

DOI  :  10.1292/jvms.15-0222

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)The effect of environmental acidification on Ibaraki virus (IBAV) infection was testedusing endosomal inhibitory chemicals and low pH treatment. Treatment of target cells withendosomal inhibitors significantly decreased the progeny virus production. IBAV outercapsid proteins, VP5 and VP2, were removed from virion when purified IBAV was exposed tolow pH environment. Further experiment showed that the exposure to low pH bufferfacilitated IBAV infection when the cellular endosomal pathway was impaired by bafilomycinA1. Results obtained in this study suggest that acidic environment is essential toinitiate IBAV infection.

【 授权许可】

Unknown