Planktonic primary production in estuaries: comparison of 14C, O2 and 18O methods

Aquatic Microbial Ecology

J. J. Middelburg¹  M. Loijens¹  J.-P. Gattuso¹  M.-D. Pizay¹  F. Gazeau¹  J.-P. Vanderborght¹ 

关键词: Primary production;    Phytoplankton;    Estuaries;    Incubation method;    Oxygen;    Carbon-14;    Oxygen-18;   

DOI  :  10.3354/ame046095

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Rates of primary production were measured in 2 estuaries (Randers Fjord, Denmark, and the Scheldt estuary, Belgium/The Netherlands) using 3 different incubation methods: (1) the oxygen light-dark method (O₂-LD), (2) ¹⁴C incorporation and (3) ¹⁸O labeling. Estimates based on the ¹⁴C incorporation technique were not significantly different from those obtained using the O₂-LD technique. The ¹⁸O approach provided rates significantly lower than the 2 other techniques. Ratios of O₂-LD to ¹⁸O-based rates (range: 0.99 to 3.54) were often statistically significantly higher than 1 and increased with decreasing salinities and/or lower oxygen concentrations. The underestimation of gross primary production by the ¹⁸O method may be due to an intracellular recycling of labeled oxygen which increased in magnitude with decreasing external oxygen conditions. These results suggest that the ¹⁸O method must be used with extreme care in nutrient-rich, low oxygen systems.

【 授权许可】

Unknown