Liver Hydrolysate Assists in the Recovery From Physical Fatigue in a Mouse Model

Journal of Pharmacological Sciences

Koichi Tan-No³  Kotaro Yamada¹  Masahiro Fukahori¹  Wataru Nemoto³  Takeshi Tadano²  Osamu Nakagawasai³ 

[1] Consumer Healthcare Products Development, Zeria Pharmaceutical Co., Ltd., Japan;Laboratory of Environmental and Health Sciences, College of Medical Pharmaceutical and Health Sciences, Kanazawa University, Japan;Department of Pharmacology, Tohoku Pharmaceutical University, Japan

关键词: liver hydrolysate;    physical fatigue;    5�?-AMP-activated protein kinase;    glycogen;    lactic acid;   

DOI  :  10.1254/jphs.13084FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(33)Cited-By(3)It is reported that liver hydrolysate (LH) enhances liver function. However, the effects of LH on physical fatigue are unknown. The aim of this study was to investigate the effect of LH on alterations in locomotor activity and energy metabolism such as 5�?-AMP-activated protein kinase (AMPK), glycogen content, and blood lactic acid, after forced walking. Adult male ddY mice were used. Locomotor activity, AMPK phosphorylation, and glycogen content in the liver and soleus muscle, as well as blood lactic acid were determined following LH treatment before and/or after forced walking. The locomotor activity significantly decreased after forced walking for 3 h. Two administrations of LH (30 or 100 mg/kg) significantly increased the locomotor activity, while a single administration either before or after forced walking did not show any specific effect. Administering LH twice activated AMPK in the liver and soleus muscle. Glycogen levels significantly decreased in both the liver and soleus muscle after forced walking, whereas the blood lactate level significantly increased. In contrast, administering LH twice increased muscle glycogen and decreased blood lactic acid. These findings indicate that LH produced an anti-fatigue effect and that this effect appears to involve the efficient glycogen utilization through activation of AMPK.

【 授权许可】

Unknown