Granulocyte Colony-Stimulating Factor Has No Adverse Effects on Atherosclerotic Lesions in High Cholesterol-Fed Miniature Swine

Journal of Veterinary Medical Science

Atsuhiko KATO¹  Kiyoka KATSUYAMA²  Masami SUZUKI¹  Etsuko FUJII¹  Tsuneo ITO²  Kenji ADACHI¹  Masaki YAMAZAKI¹  Kouichi KATAGIRI²  Akio MIYOSHI¹  Hirotake TAKAI¹  Hitoshi ARAKAWA²  Shuji HAYASHI¹ 

[1] Department of Safety Assessment, Fuji Gotemba Research Laboratory, Chugai Pharmaceutical Co., Ltd.;Chugai Research Institute for Medical Science, Inc.

关键词: atherosclerosis;    atherosclerotic lesions;    G-CSF;    high cholesterol diet;    miniature swine;   

DOI  :  10.1292/jvms.70.943

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(5)Granulocyte colony-stimulating factor (G-CSF) is widely used to mobilize peripheral blood stem cells, and expected to restore cardiac function for patients with coronary artery diseases as a consequence of progression of atherosclerosis. Safety issues related to the administration of G-CSF to these patients, however, are still under study. The animal model for atherosclerosis was produced by feeding miniature swine a high-cholesterol diet for 3 months. G-CSF (5 or 10 μg/kg/day) was given to the animal model by daily subcutaneous injections for 10 days and 20 main arteries were evaluated pathologically. In addition, the general toxicological effects were studied on clinical signs, body weight, hematology, blood chemistry and pathology. In the G-CSF-treated groups, a variety of changes related to the major pharmacological activity of G-CSF including an increase in white blood cell (WBC) counts were observed. In many arteries, atherosclerotic lesions similar to Type I-V of the proposed classification by the American Heart Association were observed. No effects of the G-CSF treatment were seen on the histopathological findings, incidence, severity or distribution of atherosclerotic lesions. In addition, no infiltration of neutrophils to the lesions was observed. These findings suggest that the administration of G-CSF causes neither exacerbation or modification of atherosclerotic lesions nor adverse changes despite that a sufficient increase in WBC counts could be achieved in the peripheral blood.

【 授权许可】

Unknown