Changes in O2– and H2O2 production by Chattonella antiqua during diel vertical migration under nutrient stratification

Aquatic Microbial Ecology

Daekyung Kim Masataka Watanabe¹  Yoko Nakayasu Kunio Kohata¹ 

关键词: Chattonella antiqua;    Diel vertical migration;    Hydrogen peroxide;    Nutrient stratification;    Reactive oxygen species;    Superoxide anion;   

DOI  :  10.3354/ame039183

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The red tide flagellate Chattonella antiqua produces high levels of reactive oxygen species (ROS) such as the superoxide anion (O₂^–) and hydrogen peroxide (H₂O₂). Production of ROS by thisflagellate species depends on nutrient and water conditions during its growth phases. To clarify the effect of nutrient depletion on ROS production, we investigated the production pattern of ROS in a culture tank containing 1 m³ of medium withlow nitrate and phosphate nutrient concentrations. The production of O₂^– and H₂O₂ by C. antiqua was lower when nutrients were limited than when they were fully supplied (f/2 medium), as indicated byreductions in nitrogen and phosphorus cell quotas. We also examined ROS formation by C. antiqua under stratified conditions subsequent to the nutrient depletion experiment. The vertical stratification of salinity (ΔS = 2.5 psu) andtemperature (ΔT = 5°C) in the tank were analogous to natural conditions during red tides, and only the bottom layer was enriched with nutrients. C. antiqua showed clear diel vertical migration under stratified conditions. The ROSproduction due to photosynthesis was high during the light period at the surface, even though the ambient nutrient concentration of the surface was low, and it decreased in the dark bottom layer. The generation of H₂O₂ increasedcontinuously for 4 d, but O₂^– generation peaked on the second day of stratification and then gradually decreased.

【 授权许可】

Unknown