Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced bycadmium, mercury and methylmercury

Journal of Veterinary Medical Science

Atsushi MIYAMOTO¹  Mitsuya SHIRAISHI¹  Masato OHKUBO¹ 

[1] Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1�?21�?24 Korimoto, Kagoshima 890�?0065, Japan

关键词: Fura-2;    heavy metal;    SH-SY5Y;    TPEN;   

DOI  :  10.1292/jvms.15-0620

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Stimulation with heavy metals is known to induce calcium (Ca2+)mobilization in many cell types. Interference with the measurement of intracellularCa2+ concentration by the heavy metals in cells loaded with Ca2+indicator fura-2 is an ongoing problem. In this study, we analyzed the effect of heavymetals on the fura-2 fluorescence ratio in human SH-SY5Y neuroblastoma cells by usingTPEN, a specific cell-permeable heavy metal chelator. Manganese chloride (30�?300µM) did not cause significant changes in the fura-2 fluorescence ratio.A high concentration (300 µM) of lead acetate induced a slight elevationin the fura-2 fluorescence ratio. In contrast, stimulation with cadmium chloride, mercurychloride or MeHg (3�?30 µM) elicited an apparent elevation of the fura-2fluorescence ratio in a dose-dependent manner. In cells stimulated with 10 or 30µM cadmium chloride, the addition of TPEN decreased the elevated fura-2fluorescence ratio to basal levels. In cells stimulated with mercury or MeHg, the additionof TPEN significantly decreased the elevation of the fura-2 fluorescence ratio induced bylower concentrations (10 µM) of mercury or MeHg, but not by higherconcentrations (30 µM). Pretreatment with Ca2+ channelblockers, such as verapamil, 2-APB or lanthanum chloride, resulted in different effects onthe fura-2 fluorescence ratio. Our study provides a characterization of the effects ofseveral heavy metals on the mobilization of divalent cations and the toxicity of heavymetals to neuronal cells.

【 授权许可】

Unknown